PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, coding gene and engineering bacterium

The invention discloses a PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, a coding gene and an engineering bacterium, the PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability is named as IsPETase S92P/D157A-cSP, the amino acid sequence of the IsPETase S92P/D157A-cSP is shown as SEQ ID NO.1, the melting temperature of the IsPETase S92P/D157A-cSP is measured to be greatly increased by a differential scanning fluorescence method, and the thermal stability of the IsPETase-cSP mutant enzyme is remarkably enhanced. When the IsPETase S92P/D157A-cSP is used for degrading the PET film, the degradation effect is obviously improved. 本发明公开了一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶及编码基因及工程菌，一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶，命名为IsPETaseS92P/D157A-cSP，所述IsPETaseS92P/D157A-cSP的氨基酸序列如SEQ ID NO.1所示，差式扫描荧光法测得其熔融温度大幅提升，热稳定性显著增强。使用IsPETaseS92P/D157A-cSP降解PET膜，降解效果显著提高。