Poly-3-hydroxybutyrate recombinant shewanella strain synthesized by formic acid and construction method and application thereof

The invention discloses a recombinant shewanella bacterial strain for synthesizing poly-3-hydroxybutyrate by utilizing formic acid as well as a construction method and application of the recombinant shewanella bacterial strain, and the construction method comprises the following steps: introducing a...

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Bibliographische Detailangaben
Hauptverfasser:	SONG HAO, WANG YE, DOU GUANGJIN, TIAN YAO, GUO ZHIQI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a recombinant shewanella bacterial strain for synthesizing poly-3-hydroxybutyrate by utilizing formic acid as well as a construction method and application of the recombinant shewanella bacterial strain, and the construction method comprises the following steps: introducing a gene FTL containing a source of Saccharomyces cerevisiae S288C into a wild type Shewanella halofaensis through an FTLpHG12-pBAD plasmid, so as to obtain a recombinant bacterial strain Sh01 by utilizing formic acid. The method comprises the following steps: introducing synthetic genes phaA, phaB1 and phaC1 containing poly (3-hydroxybutyrate) from Ralstinia eutropha H16 into Sh01 through a plasmid phaABC-FtL-pHG12-pBAD, and constructing a shewanella bacterial strain Sh02 for synthesizing the poly (3-hydroxybutyrate) (PHB) by utilizing formic acid. The method for synthesizing the poly-3-hydroxybutyrate by utilizing formic acid and seawater fermentation through the microorganism is simple and convenient to operate, an