Construction method of cell tissue bank of placenta tissue as well as related product and application of cell tissue bank

The invention discloses a construction method of a cell tissue bank of placenta tissue and related products and application thereof, and relates to the technical field of cell tissue bank construction.The equilibrium liquid and cryopreservation liquid are prepared from 5-300 mM saccharose, 0.5%-5% b...

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Bibliographische Detailangaben
Hauptverfasser: REN YUQIANG, WU XIAOYUN, WANG LIMING, BAO HONGLEI, TIAN ZHIHAI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a construction method of a cell tissue bank of placenta tissue and related products and application thereof, and relates to the technical field of cell tissue bank construction.The equilibrium liquid and cryopreservation liquid are prepared from 5-300 mM saccharose, 0.5%-5% by volume of glycerinum, 2.5-25 mM creatine, 2.5-25 mM isoleucine, 25-250 mM mannitol and 5%-20% by mass of albumin or serum, the mass fraction of the dextran 40 sodium chloride injection is 30%-80%. The cryopreservation liquid and the equilibrium liquid provided by the invention can effectively reduce the damage of direct freezing to placenta tissue blocks and cells thereof, improve the yield and efficiency of cell resuscitation, and effectively protect all cell types in placenta and various bioactive substances such as nucleic acid and protein molecules in placenta tissues; and useful value resources in the placenta are preserved to the greatest extent. 本发明公开了一种胎盘组织的细胞组织库的构建方法及其相关产品和应用,涉及细胞组织库构建的技术领域,所述平衡液和冻存液包括:5