High-specificity and high-sensitivity immunochromatography detection test paper, preparation method thereof, detection kit and detection method thereof

The invention discloses immunochromatography test paper with high specificity and high sensitivity. The immunochromatography test paper comprises a conjugate pad I, a conjugate pad II, a nitrocellulose membrane coated with a C line and a T line, a sample suction pad and a bottom plate, wherein an antibody A fixed with an antigen to be detected is coupled with oxidized dextran; conA-coupling colloidal gold and anti-DNP antibody coupling colloidal gold are fixed on the second combination pad; the T line is coated with an antibody B of an antigen to be detected; and the C line is coated with DNP. The invention also discloses a preparation method of the test paper, a detection kit and a detection method. Glucan and a ConA system are introduced, ConA is tetramer globulin, each subunit contains one sugar binding site, and each ConA has four sugar binding sites, so that a detection signal is enhanced, signal amplification is realized, and the detection sensitivity is improved. The method is suitable for detection of