Nucleic acid aptamer capable of being specifically combined with PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) N protein and application of nucleic acid aptamer

The invention provides a nucleic acid aptamer of an N protein of a porcine reproductive and respiratory syndrome virus (PRRSV) and application of the nucleic acid aptamer. Candidate nucleic acid aptamers aiming at PRRSV N protein are screened from a nucleic acid library by utilizing an affinity chromatography column method, the aptamers with higher repetition rate are selected through sequencing comparison, and the aptamers are subsequently evaluated through sequence analysis, secondary structure prediction, affinity and specificity determination and the like. Finally, two PRRSV N protein nucleic acid aptamers with excellent binding force and specificity are obtained, and a favorable technical support is provided for the development of a subsequent PRRSV detection technology and the establishment of an enzyme-linked aptamer method. 本发明提供一种猪繁殖与呼吸综合征病毒PRRSV的N蛋白的核酸适配体及其用途。本发明利用亲和层析柱法，从核酸文库中筛选出了针对PRRSV N蛋白的候选核酸适配体，通过测序比对，选出了重复率较高的适配体，后续通过序列分析、二级结构预测、亲和力和特异性测定等对适配体进行评价，最终获得了两条结合力和特异性极好的PRRSV N蛋白核酸适配体，为后续PRRSV的检测技术