Stem cell separation device and separation method

The invention relates to the technical field of separation devices, and provides a stem cell separation device and a separation method, the aim of retaining stem cells is achieved by adding antibodies, combining complement with the toxic killing effect on cells and removing other active cells according to the principle that a blastocyst cavity is impermeable to antibodies, meanwhile, adding is uniform, and the separation efficiency is high. The spraying device and a culture vessel for stem cell culture are relatively easy to assemble and disassemble, the spraying device comprises a sprinkling can, a vessel and the spraying device, the vessel is detachably provided with a sealing cover, the sealing cover is provided with a spiral track pipe, a follow-open cover is installed in the track pipe, the spraying device comprises a spiral moving cylinder, the spiral moving cylinder is communicated with a quick connecting pipe, and the quick connecting pipe is communicated with the quick connecting pipe. The quick conn