Synthesis method of platelet nanomotor loaded with gold nanoparticles in situ

The invention discloses a synthesis method of a platelet nanomotor loaded with gold nanoparticles in situ. Comprising the following steps: preparing a platelet suspension, preparing PLT-coated Au, and synthesizing a platelet nano motor urea-biotin loaded with gold nanoparticles in situ. The platelet nano-motor driven by urease loaded with gold nanoparticles in situ is constructed, and the synthesized nano-motor still retains the original functional characteristics of platelets, such as specific binding capacity. Under the action of urea fuel, the concentration gradient generated by the catalytic reaction of urease on the surface of platelets and the urea fuel can significantly promote the uptake of the nanoparticles by tumor cells. 本发明公开了一种原位负载金纳米颗粒的血小板纳米马达的合成方法。包括以下步骤：制备血小板悬浮液、制备PLT@Au、原位负载金纳米颗粒的血小板纳米马达urease-biotin合成。本发明构建了一个原位负载金纳米颗粒脲酶驱动的血小板纳米马达，合成的纳米马达仍保留血小板原有的功能特性，如特异性结合能力。在尿素燃料作用下，血小板表面的尿素酶与其发生催化反应产生的浓度梯度可以显著促进肿瘤细胞对纳米颗粒的摄取。