Porcine A type foot-and-mouth disease mRNA vaccine and preparation method thereof
The invention belongs to the field of foot-and-mouth disease vaccines, and particularly relates to a swine A type foot-and-mouth disease mRNA vaccine and a preparation method thereof. The preparation method comprises the following steps: by taking a porcine A type foot and mouth disease virus (Re-A/...
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| creator | DONG JINJIE WANG FAN ZHI XIAOYING YANG JINCAI LIU PING DENG RUIXUE CHANG YANYAN ZHANG KESHAN WANG HUIBAO YANG RUI |
| description | The invention belongs to the field of foot-and-mouth disease vaccines, and particularly relates to a swine A type foot-and-mouth disease mRNA vaccine and a preparation method thereof. The preparation method comprises the following steps: by taking a porcine A type foot and mouth disease virus (Re-A/WH/09 strain) inactivated antigen as a template, amplifying a structural protein VP1 gene, and connecting the amplified structural protein VP1 gene with a pSFV1 vector to construct a recombinant plasmid pSFV1-VP1; the mRNA obtained by treating recombinant plasmids through methods such as linearization and in-vitro transcription is mixed with the prepared lipidosome nanoparticles to prepare the foot-and-mouth disease mRNA vaccine; after the guinea pig is immunized by the mRNA vaccine, the neutralizing antibody, the IL-4 and the IFN-gamma are obviously higher than those of a negative control group; immune protection can be provided for Re-A/WH/09 strain attack of 50 times of GPID50. Therefore, the mRNA vaccine prepar |
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The preparation method comprises the following steps: by taking a porcine A type foot and mouth disease virus (Re-A/WH/09 strain) inactivated antigen as a template, amplifying a structural protein VP1 gene, and connecting the amplified structural protein VP1 gene with a pSFV1 vector to construct a recombinant plasmid pSFV1-VP1; the mRNA obtained by treating recombinant plasmids through methods such as linearization and in-vitro transcription is mixed with the prepared lipidosome nanoparticles to prepare the foot-and-mouth disease mRNA vaccine; after the guinea pig is immunized by the mRNA vaccine, the neutralizing antibody, the IL-4 and the IFN-gamma are obviously higher than those of a negative control group; immune protection can be provided for Re-A/WH/09 strain attack of 50 times of GPID50. 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The preparation method comprises the following steps: by taking a porcine A type foot and mouth disease virus (Re-A/WH/09 strain) inactivated antigen as a template, amplifying a structural protein VP1 gene, and connecting the amplified structural protein VP1 gene with a pSFV1 vector to construct a recombinant plasmid pSFV1-VP1; the mRNA obtained by treating recombinant plasmids through methods such as linearization and in-vitro transcription is mixed with the prepared lipidosome nanoparticles to prepare the foot-and-mouth disease mRNA vaccine; after the guinea pig is immunized by the mRNA vaccine, the neutralizing antibody, the IL-4 and the IFN-gamma are obviously higher than those of a negative control group; immune protection can be provided for Re-A/WH/09 strain attack of 50 times of GPID50. 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The preparation method comprises the following steps: by taking a porcine A type foot and mouth disease virus (Re-A/WH/09 strain) inactivated antigen as a template, amplifying a structural protein VP1 gene, and connecting the amplified structural protein VP1 gene with a pSFV1 vector to construct a recombinant plasmid pSFV1-VP1; the mRNA obtained by treating recombinant plasmids through methods such as linearization and in-vitro transcription is mixed with the prepared lipidosome nanoparticles to prepare the foot-and-mouth disease mRNA vaccine; after the guinea pig is immunized by the mRNA vaccine, the neutralizing antibody, the IL-4 and the IFN-gamma are obviously higher than those of a negative control group; immune protection can be provided for Re-A/WH/09 strain attack of 50 times of GPID50. Therefore, the mRNA vaccine prepar</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Porcine A type foot-and-mouth disease mRNA vaccine and preparation method thereof |
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