Porcine A type foot-and-mouth disease mRNA vaccine and preparation method thereof

The invention belongs to the field of foot-and-mouth disease vaccines, and particularly relates to a swine A type foot-and-mouth disease mRNA vaccine and a preparation method thereof. The preparation method comprises the following steps: by taking a porcine A type foot and mouth disease virus (Re-A/WH/09 strain) inactivated antigen as a template, amplifying a structural protein VP1 gene, and connecting the amplified structural protein VP1 gene with a pSFV1 vector to construct a recombinant plasmid pSFV1-VP1; the mRNA obtained by treating recombinant plasmids through methods such as linearization and in-vitro transcription is mixed with the prepared lipidosome nanoparticles to prepare the foot-and-mouth disease mRNA vaccine; after the guinea pig is immunized by the mRNA vaccine, the neutralizing antibody, the IL-4 and the IFN-gamma are obviously higher than those of a negative control group; immune protection can be provided for Re-A/WH/09 strain attack of 50 times of GPID50. Therefore, the mRNA vaccine prepar