Method for improving female lamb rate of offspring of milk goats

The method comprises the following steps: firstly, determining specifically expressed protein DPY30 in Y sperms of the milk goats, then packaging goat DPY30 gene interference lentivirus, injecting the goat DPY30 gene interference lentivirus into testis of a stud ram, collecting semen of the stud ram...

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Hauptverfasser: SHI HUAIPING, WANG PENGFEI, ZHANG HUIJUN, LI JIE, BIAN HUILONG, LUO JUN, HE HUANSHAN, LI CONG, LI JINTAO
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creator SHI HUAIPING
WANG PENGFEI
ZHANG HUIJUN
LI JIE
BIAN HUILONG
LUO JUN
HE HUANSHAN
LI CONG
LI JINTAO
description The method comprises the following steps: firstly, determining specifically expressed protein DPY30 in Y sperms of the milk goats, then packaging goat DPY30 gene interference lentivirus, injecting the goat DPY30 gene interference lentivirus into testis of a stud ram, collecting semen of the stud ram after 47d, identifying the semen quality and the proportion of the X sperms and the Y sperms in upstream sperms, and determining the female lamb rate of the offspring of the milk goats according to the semen quality and the proportion of the X sperms and the Y sperms. After artificial insemination with the semen of the breeding ram injected with the DPY30 gene interference lentivirus, the female lamb rate reaches 58.20% and is increased by 22.08%, and the number of born female lambs is remarkably increased. The interference lentivirus is adopted to regulate and control expression of the gene DPY30 of the specific expression protein in the Y sperm of the coded milk goat in the goat testis, the female lambing rate o
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language chi ; eng
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
VINEGAR
WINE
title Method for improving female lamb rate of offspring of milk goats
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