Protein chip confining liquid based on polystyrene material and preparation method of protein chip confining liquid

The invention discloses a protein chip confining liquid based on a polystyrene material and a preparation method of the protein chip confining liquid. The protein chip confining liquid based on the polystyrene material is prepared from the following components: 1 g/100 ml to 10 g/100 ml of bovine se...

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Hauptverfasser: WANG YING, XI ZAIJUN, WAN SHUAISHUAI, JI HAIPENG, YANG SHOUSHENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a protein chip confining liquid based on a polystyrene material and a preparation method of the protein chip confining liquid. The protein chip confining liquid based on the polystyrene material is prepared from the following components: 1 g/100 ml to 10 g/100 ml of bovine serum albumin, 2 g/100 ml to 12 g/100 ml of cane sugar, 0.01 vol% to 0.03 vol% of PC300 and 0.005 to 0.015 M of buffer solution. According to the formula of the confining liquid provided by the invention, not only can the confining effect on bioactive substances be achieved, but also the signal-to-noise ratio of chip detection can be increased, and the uniformity of chip detection can be improved; the confining liquid provided by the invention is simple in preparation method, low in cost, free of environmental pollution and non-toxic and harmless to a human body. 本发明公开了一种基于聚苯乙烯材质的蛋白芯片封闭液及其制备方法。该基于聚苯乙烯材质的蛋白芯片封闭液,其由如下组分组成:1g/100ml~10g/100ml牛血清白蛋白、2g/100ml~12g/100ml蔗糖、0.01vol%~0.03vol%的PC300、0.005~0.015M缓冲液。本发明提供的封闭液配方,