System suitable for genome evolution of Rorsella general formula bacteria, application and genome evolution method

The invention discloses a system suitable for genome evolution of Ralls general formula bacteria, application and a genome evolution method. The system suitable for the genome evolution of the Ralsa general formula bacteria comprises an expression vector for carrying out fusion expression on a gene for coding cytosine deaminase and a gene for coding a specific single-stranded DNA binding protein. The genome evolution method established by the invention has the advantages of simplicity, high efficiency and the like in operation when being used for continuously evolving the genome of the Ralls general formula bacteria; functionally, the substrate consumption rate can be increased, the substrate spectrum can be expanded, the tolerance to growth environments such as an organic reagent, an oxidizing agent and an electric microorganism system can be enhanced, and subsequent synthetic biological modification is facilitated. Therefore, by using the genome evolution method disclosed by the invention, the diversity of