Preparation method of split influenza virus vaccine

The invention provides a preparation method of an influenza virus split vaccine, which comprises the following steps: carrying out serum-free full-suspension enlarged culture on canine kidney cells MDCK-XF04 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2018192; the method comprises the following steps: diluting canine kidney cells MDCK-XF04 by using an MDCK cell maintenance culture medium, inoculating influenza virus for culture proliferation, and harvesting influenza virus liquid; preparing the influenza virus liquid into a monovalent stock solution of influenza virus; and preparing the univalent stock solution into the influenza virus split vaccine. The production efficiency is high; the production scale is easy to enlarge, the product uniformity is good, and the batch-to-batch difference is small; the cell culture density is high, the influenza virus proliferation titer is high, the passage cell proliferation virus is not easy to vary, and the vaccine is more effect