Method for removing bacterial contamination in hybridoma cells

The invention discloses a method for removing bacterial contamination in hybridoma cells, and the method for removing bacterial contamination in hybridoma cells comprises the following steps: centrifuging the hybridoma cells with bacterial contamination, discarding the supernatant, adding a culture medium, and resuspending to obtain resuspended hybridoma cells; injecting the resuspended hybridoma cells into the abdominal cavity of a mouse to obtain an experimental mouse, culturing, and taking out liquid in the abdominal cavity of the mouse to obtain first hybridoma cells; transferring the first hybridoma cell into a sterile centrifuge tube, centrifuging, discarding the supernatant, resuspending the cell precipitate by using a culture medium, and paving the cell suspension in a plate hole for culturing to obtain a second hybridoma cell; carrying out subcloning on the second hybridoma cell to obtain a subcloned cell; and carrying out amplification culture on the subcloned cells to obtain purified hybridoma cell