Yeast cytosine deaminase fusion protein as well as mutant and application thereof

The invention belongs to the field of molecular biology, and particularly relates to a yeast cytosine deaminase fusion protein as well as a mutant and application thereof. According to the invention, related researches are developed on how to improve the catalytic activity of the yCD, so that the yCD mutant with high catalytic activity is screened, data faults for improving the catalytic activity of the yCD can be filled up, a related research basis is provided for comprehensive application of a 5-FC/yCD system in the field of cancer treatment, a more excellent alternative is provided for clinical application of the 5-FC/yCD system, and the application of the 5-FC/yCD system in the field of cancer treatment is facilitated. And the clinical application process of cancer targeted therapy is accelerated. 本发明属于分子生物学领域，具体涉及一种酵母胞嘧啶脱氨酶融合蛋白及其突变体和应用。本发明就如何提高yCD催化活性开展相关研究，以筛选到高催化活性的yCD突变体，有利于填补提高yCD催化活性的数据断层，为5-FC/yCD系统在癌症治疗领域的全面应用提供相关的研究基础，为5-FC/yCD系统的临床应用提供更优良的备选，加速癌症靶向治疗的临床应用进程。