Purification of alpha-1 proteinase inhibitor using novel chromatographic separation conditions

本发明涉及用在pH低于6.0和低离子强度下溶液的阳离子色谱从生物流体包括血浆和血浆组分中提纯人α-1蛋白酶抑制剂(α-1PI)的方法。阳离子色谱的优点在于：在这些条件下活性α-1PI不与该阳离子柱结合而其它蛋白质包括变性的α-1PI和白蛋白与该阳离子柱结合。结果，活性α-1PI流过该色谱柱，而污染蛋白质留在柱上。α-1PI的回收率高，且纯度的提高是惊人的。 Cation chromatography with solutions at pH less than 6.0 and low ionic strength can be utilized to purify human alpha -1 proteinase inhibitor ( alpha -1 PI) from biological fluids including plasma and plasma fractions. The cation chromatography takes advantage of the fact that active alpha -1 PI does not bind to the cation column under these conditions but other proteins, including denatured alpha -1 PI and albumin, do. The effect is that active alpha -1 PI flows through the chromatography column leaving the contaminating proteins behind. Recovery of alpha -1 PI is high and improvement of purity is dramatic.