Method for simultaneously determining content of vitamin ADE in formula food
The invention relates to a method for simultaneously determining the content of vitamin ADE in formula food, and relates to the field of food analysis, and the method comprises the following steps: step 1, pretreatment of formula food to be determined, step 2, determination of a detection liquid of...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a method for simultaneously determining the content of vitamin ADE in formula food, and relates to the field of food analysis, and the method comprises the following steps: step 1, pretreatment of formula food to be determined, step 2, determination of a detection liquid of the formula food to be determined: adopting an ultra-performance liquid chromatograph, adopting a C18 and PFP series chromatographic column, taking methanol-water as a mobile phase, and carrying out gradient elution on the detection liquid of the formula food to be determined; an ultraviolet detector and a fluorescence detector are connected in series, and the vitamin A, the vitamin D2, the vitamin D3, the vitamin alpha-VE, the vitamin beta-VE, the vitamin gamma-VE and the vitamin delta-VE are qualitatively or/and quantitatively detected at the same time. C18 and PFP series chromatographic columns are adopted, simultaneous separation of vitamin A, vitamin D (D2 and D3) and vitamin E (alpha-VE, beta-VE, gamma-VE and |
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