Method for detecting trace biguanide preservative in eye care solution containing sodium hyaluronate

The invention relates to a method for detecting trace biguanide preservatives in a sodium hyaluronate-containing eye care solution. The method comprises the following steps: A, adding sodium hyaluronate enzyme into a to-be-detected sample solution for enzymolysis; and B, directly injecting the to-be-detected sample solution subjected to enzymolysis into a high performance liquid chromatograph, eluting by adopting a CS-C18 chromatographic column and taking a buffer salt solution with the pH value of 3.5-6.0 and acetonitrile as mobile phases, and detecting the content of the biguanide preservative. The method can realize accurate detection of trace biguanide preservatives in the contact lens care solution, and has the advantages of good linearity, high recovery rate, short analysis time, conventional and easily available consumables, economy and high efficiency. 本申请涉及一种含透明质酸钠的眼用护理液中的微量双胍类防腐剂的检测方法，包括以下步骤：A、向待测样品溶液加入透明质酸钠酶进行酶解；B、将酶解后的待测样品溶液直接进样至高效液相色谱仪中，采用CS-C18色谱柱，以pH值为3.5～6.0的缓冲盐溶液与乙腈作为流动相进行洗脱，检测双胍类防腐剂含量。该方法可实现