Rapid HLA typing method
The invention belongs to the technical field of biology, and particularly relates to a rapid HLA typing method. The invention discloses a rapid HLA (human leukocyte antigen) typing method which comprises the following steps: S1, preparing a linearized cloning vector, and purifying to obtain the line...
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Zusammenfassung: | The invention belongs to the technical field of biology, and particularly relates to a rapid HLA typing method. The invention discloses a rapid HLA (human leukocyte antigen) typing method which comprises the following steps: S1, preparing a linearized cloning vector, and purifying to obtain the linearized cloning vector; s2, respectively carrying out sample PCR amplification by adopting specific primer pairs of different HLA gene exons, and purifying to obtain respective HLA gene exon PCR amplification products; s3, respectively constructing recombinant vectors of different HLA gene exons; s4, transforming a recombinant vector; s5, screening positive strains; s6, sequencing and comparing the sequence with a standard sequence of an HLA gene exon in a database to determine the type of the HLA gene; the method is characterized in that in the step S4 of transforming the recombinant vector, transformation is carried out according to the proportion that the closest competent cell number/recombinant vector DNA mole |
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