Freeze-drying protective agent and application thereof in preparation of PCR (polymerase chain reaction) freeze-drying reagent

The invention relates to the technical field of molecular biology, in particular to a freeze-drying protective agent and application of the freeze-drying protective agent in preparation of a PCR freeze-drying reagent. According to the freeze-drying protective agent composition containing the saccharides and the tert-butyl alcohol, the water content of a PCR freeze-drying reagent can be reduced in a common low-humidity environment, and the moisture resistance is improved. The freeze-dried microspheres obtained through the preparation method are high in hardness, scraps are not prone to falling off, and manual and automatic split charging is facilitated. 本发明涉及分子生物技术领域，尤其涉及冻干保护剂及其在制备PCR冻干试剂中的应用。本发明提供的含有糖类和叔丁醇的冻干保护剂组合，能够使PCR冻干试剂在普通低湿环境中减少含水量，提高耐湿性。利用本发明所述制备方法获得的冻干微球硬度高、不易掉屑，有利于人工和自动化分装。