Preparation method and application of recombinant cystathionine-beta-lyase

The invention discloses a preparation method and application of recombinant cystathionine-beta-lyase. According to the preparation method of the recombinant cystathionine-beta-lyase based on the prokaryotic expression system, synonymous codon preference optimization is performed on a gene sequence f...

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Bibliographische Detailangaben
Hauptverfasser:	ZHU WEIWEI, LIU CHUNQI, LEI YONGSEN, LIU WENLI, JIANG XIWEN, CHEN YUANYUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a preparation method and application of recombinant cystathionine-beta-lyase. According to the preparation method of the recombinant cystathionine-beta-lyase based on the prokaryotic expression system, synonymous codon preference optimization is performed on a gene sequence for coding the cystathionine-beta-lyase in different modes, the obtained optimized codon constructs a carrier, a large amount of soluble protein can be stably expressed in escherichia coli, and the recombinant cystathionine-beta-lyase can be used for preparing the recombinant cystathionine-beta-lyase. The yield is increased, the purification step is simplified, and the produced recombinant cystathionine-beta-lyase has high activity and is suitable for industrial production. 本申请公开了一种重组胱硫醚-β-裂解酶的制备方法和应用。本申请中提供了基于原核表达体系的重组胱硫醚-β-裂解酶制备方法，通过对编码胱硫醚-β-裂解酶的基因序列进行不同方式的同义密码子偏好性优化，所得优化密码子构建了载体，在大肠杆菌中可稳定表达大量可溶蛋白，提高了产量并简化了纯化步骤，产出的重组胱硫醚-β-裂解酶活性高，适宜工业化生产。