Human G9 type rotavirus HN-28 strain and application thereof

The invention discloses a human G9 type rotavirus HN-28 strain which is preserved in the China Center for Type Culture Collection on January 13, 2022, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: V202203. RNA-PAGE shows that the electrophoresis type of the virus strain is long, and 11 bands are arranged in a unique electrophoretogram 4: 2: 3: 2 of the group A rotavirus; the whole genome next-generation sequencing result shows that the strain is the G9P [8] type rotavirus. According to the invention, a G9 type rotavirus wild strain capable of stable passage is successfully separated through isolated culture and cloning purification of a rotavirus positive fecal sample, and the G9 type rotavirus wild strain is named as an HN-28 strain and can be used for preparing rotavirus vaccines. 本发明公开了一株人G9型轮状病毒HN-28株，该毒株已于2022年1月13日保藏于中国典型培养物保藏中心，保藏编号为：CCTCC NO:V202203。RNA-PAGE显示该病毒株电泳型为长型，11条带呈现A组轮状病毒独特的电泳图谱4：2：3：2排列；全基因组二代测序结果显示此毒株为G9P[8]型轮状病毒。本发明通过对轮状病毒阳性粪样的分离培养和克隆纯化，成功分离了一株能稳定传代的