Preparation method of exosome and cell culture box thereof

The invention provides a preparation method of an exosome, and belongs to the field of exosome production, and the preparation method of the exosome comprises the specific steps of sample sampling, cell culture, separation pretreatment, ultra-high-speed separation, exosome raw material treatment, raw material detection, exosome purification and exosome subpackaging. A reasonable preparation process is provided, exosome raw materials can be detected by observing cells after culture, ensuring the quality of prepared samples and matching with raw material detection steps, the quality of the exosome after preparation is ensured, and the preparation quality of the exosome can be further improved after multiple times of purification, separation and collection. And data recorded after detection is uploaded, and the preparation process of each batch of exosomes can be conveniently inquired in cooperation with a traceability system of a computer. 本发明提供一种外泌体的制备方法，属于外泌体生产领域，该外泌体的制备方法具体步骤为样品取样、细胞培养、分离预处理、超高速分离、外泌体原料处理、原料