Monoclonal antibody of anti-HBsAg protein as well as cell strain, preparation method and application of monoclonal antibody

The invention relates to a monoclonal antibody capable of recognizing a human HBsAg antigen, a secretory cell strain, a preparation method of the monoclonal antibody and application of the secretory cell strain in immunodetection. According to the technical scheme, the mouse hybridoma cell strain 2B4 capable of efficiently secreting the anti-HBsAg protein monoclonal antibody and the anti-HBsAg protein monoclonal antibody secreted by the cell strain are obtained by selecting natural HBsAg protein to immunize a mouse and carrying out cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing HBsAg protein, and is suitable for immunological detection, especially immunohistochemical detection. 本发明涉及一种可以识别人HBsAg抗原的单克隆抗体，分泌细胞株、其制备方法以及其在免疫检测中的用途。上述技术方案选取天然HBsAg蛋白对小鼠进行免疫，经细胞融合、筛选和亚克隆，获得高效分泌抗HBsAg蛋白单克隆抗体的小鼠杂交瘤细胞株2B4以及由该细胞株所分泌的抗HBsAg蛋白单克隆抗体。本方案得到的抗体具有高特异性、敏感性，可以特异性识别表达HBsAg蛋白的细胞，适用于免疫学检测，特别是免疫组化检测。