Biotin and antibody coupling ratio detection kit and application thereof
The invention discloses a biotin and antibody coupling ratio detection kit and application thereof, the biotin and antibody coupling ratio detection kit comprises an antibody capture carrier, a biotinylated antibody quality control product L, a biotinylated antibody quality control product H and che...
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| Format: | Patent |
| Sprache: | chi ; eng |
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| Zusammenfassung: | The invention discloses a biotin and antibody coupling ratio detection kit and application thereof, the biotin and antibody coupling ratio detection kit comprises an antibody capture carrier, a biotinylated antibody quality control product L, a biotinylated antibody quality control product H and chemiluminescence labeled streptavidin, the antibody capture carrier is Protein A/G magnetic beads or Protein A and Protein G mixture magnetic beads, and the chemiluminescence labeled streptavidin is a chemiluminescence labeled streptavidin. The coupling molar ratio of biotin to antibody of the biotinylated antibody quality control product L and the biotinylated antibody quality control product H is (1: 1)-(5: 1) and (15: 1)-(30: 1) respectively. The detection kit is wide in detection range of the coupling ratio of the biotin to the antibody, the coupling ratio of the biotin to the antibody can be detected to be 1-40, the sensitivity is high, the sample dosage is smaller than 5 microliters, and the detection kit can b |
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