DCAPS molecular marker related to watermelon seed size and application of dCAPS molecular marker

The invention provides a dCAPS molecular marker related to the size of watermelon seeds and application of the dCAPS molecular marker, and belongs to the technical field of watermelon breeding, the dCAPS molecular marker comprises an upstream primer N022-dCAPS6F with the nucleotide sequence as shown...

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Hauptverfasser: JIA YUNHE, FU YONGKAI, YAN WEN, YOU HAIBO, WANG XIQING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a dCAPS molecular marker related to the size of watermelon seeds and application of the dCAPS molecular marker, and belongs to the technical field of watermelon breeding, the dCAPS molecular marker comprises an upstream primer N022-dCAPS6F with the nucleotide sequence as shown in SEQ ID NO.1 and a downstream primer N022-dCAPS6R with the nucleotide sequence as shown in SEQ ID NO.2; the dCAPS molecular marker is designed and obtained based on a specific SNP site, when the genotype of the SNP site is GG homozygous or T/G heterozygous, the length of a watermelon seed is smaller than 10.5 mm, and when the genotype of the SNP site is TT homozygous, the length of the watermelon seed is larger than 10.5 mm. The dCAPS molecular marker can be used for accurately identifying the size of the watermelon seed, is rapid and effective, and can be applied to production on a large scale. 本发明提供了一种与西瓜种子大小相关的dCAPS分子标记及其应用,属于西瓜育种技术领域,所述dCAPS分子标记,包括核苷酸序列如SEQ ID NO.1所示的上游引物N022-dCAPS_S6F和核苷酸序列如SEQ ID NO.2所示的下游