Molecular identification and screening method of rice material with high amylose content

The invention belongs to the technical field of agricultural biological breeding, and discloses a molecular identification and screening method of a rice high-amylose-content material, which comprises the following steps: transplanting rice single plants, and extracting total DNA (Deoxyribose Nucleic Acid) of labeled single plant leaves by utilizing a CTAB (Cetyltrimethyl Ammonium Bromide) method; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification reaction by utilizing a specific primer wx-AATT of a rice germplasm with high amylose content; and carrying out gel electrophoresis and silver staining detection on a PCR amplification reaction product by using 8% non-denaturing polyacrylamide (29: 1). Photographing by utilizing a Bio-RadGeoDocXR and gel imaging system, and detecting a PCR (Polymerase Chain Reaction) amplification product and an electrophoresis result; the rice material of which the molecular weight of the PCR amplification product is 104bp is the hi