Real-time fluorescent PCR (Polymerase Chain Reaction) specific primer and probe combination for helicobacter pylori vacA genotyping

The invention discloses a real-time fluorescent PCR (Polymerase Chain Reaction) specific primer and probe combination for genotyping of helicobacter pylori vacA. The real-time fluorescent PCR specific primer and probe combination comprises a primer vacAs-F, a primer vacAs-R, a primer vacAm1-F, a primer vacAm1-R, a primer vacAm2-F, a primer vacAm2-R, a probe vacAs1-P, a probe vacAs2-P, a probe vacAm1-P and a probe vacAm2-P, the invention also provides a method and a kit for detecting the genotyping of the helicobacter pylori vacuolar toxin. According to the detection method established by the invention, the helicobacter pylori vacA gene can be typed well, and the four genes s1, s2, m1 and m2 can be detected in one time in the same system. 本发明公开了用于幽门螺杆菌vacA基因分型的实时荧光PCR特异性引物和探针组合，包括：引物vacAs-F、引物vacAs-R、引物vacAm1-F、引物vacAm1-R、引物vacAm2-F、引物vacAm2-R和探针vacAs1-P、探针vacAs2-P、探针vacAm1-P、探针vacAm2-P；本发明还提供了幽门螺杆菌空泡毒素基因分型检测的方法和试剂盒。本发明所建立的检测方法能够很好的对幽门螺杆菌vacA基因分型，并且能够在同一个体系中一次性做到s1、s2、m1、m2四种基因的检测。