(+) gamma-lactamase gene cloning and expression method and application thereof
The invention belongs to the field of gene engineering, and discloses a cloning and expression method and application of a (+) gamma-lactamase gene. A gene for producing (+) gamma-lactamase is explored by adopting a strain, target gene amplification is carried out on the gene, and an engineering bac...
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| creator | WANG CONG LIU CHUNYING CHI XUEMEI XIA SHUIYING CHI NAIYU YU SHUANG GUO XINZHI YUAN WENTAO ZHANG QINGFANG WANG YAN |
| description | The invention belongs to the field of gene engineering, and discloses a cloning and expression method and application of a (+) gamma-lactamase gene. A gene for producing (+) gamma-lactamase is explored by adopting a strain, target gene amplification is carried out on the gene, and an engineering bacterium is constructed for producing (+) gamma-lactamase, so that industrial production of (-) gamma-lactam is carried out.
本发明属于基因工程领域，公开了一种(+)γ-内酰胺酶基因的克隆与表达方法及其应用。采用菌株发掘出产(+)γ-内酰胺酶的基因，将其进行目的基因扩增，构建工程菌，用来生产(+)γ-内酰胺酶，从而进行工业化生产(-)γ-内酰胺。 |
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本发明属于基因工程领域，公开了一种(+)γ-内酰胺酶基因的克隆与表达方法及其应用。采用菌株发掘出产(+)γ-内酰胺酶的基因，将其进行目的基因扩增，构建工程菌，用来生产(+)γ-内酰胺酶，从而进行工业化生产(-)γ-内酰胺。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230512&DB=EPODOC&CC=CN&NR=116103321A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230512&DB=EPODOC&CC=CN&NR=116103321A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG CONG</creatorcontrib><creatorcontrib>LIU CHUNYING</creatorcontrib><creatorcontrib>CHI XUEMEI</creatorcontrib><creatorcontrib>XIA SHUIYING</creatorcontrib><creatorcontrib>CHI NAIYU</creatorcontrib><creatorcontrib>YU SHUANG</creatorcontrib><creatorcontrib>GUO XINZHI</creatorcontrib><creatorcontrib>YUAN WENTAO</creatorcontrib><creatorcontrib>ZHANG QINGFANG</creatorcontrib><creatorcontrib>WANG YAN</creatorcontrib><title>(+) gamma-lactamase gene cloning and expression method and application thereof</title><description>The invention belongs to the field of gene engineering, and discloses a cloning and expression method and application of a (+) gamma-lactamase gene. A gene for producing (+) gamma-lactamase is explored by adopting a strain, target gene amplification is carried out on the gene, and an engineering bacterium is constructed for producing (+) gamma-lactamase, so that industrial production of (-) gamma-lactam is carried out.
本发明属于基因工程领域，公开了一种(+)γ-内酰胺酶基因的克隆与表达方法及其应用。采用菌株发掘出产(+)γ-内酰胺酶的基因，将其进行目的基因扩增，构建工程菌，用来生产(+)γ-内酰胺酶，从而进行工业化生产(-)γ-内酰胺。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjL0KwjAURrM4iPoO102RgjHgLkVx6uReLunXNJDchCaDj-8PPoDTgcPhLFW3O-zJcYzcBLaVIxeQg4BsSOLFEctAeOYZpfgkFFGnNHwt5xy85frRdcKMNK7VYuRQsPlxpba366O9N8ipR8ls3-vat53WZ3005qQv5p_mBZ5ENdE</recordid><startdate>20230512</startdate><enddate>20230512</enddate><creator>WANG CONG</creator><creator>LIU CHUNYING</creator><creator>CHI XUEMEI</creator><creator>XIA SHUIYING</creator><creator>CHI NAIYU</creator><creator>YU SHUANG</creator><creator>GUO XINZHI</creator><creator>YUAN WENTAO</creator><creator>ZHANG QINGFANG</creator><creator>WANG YAN</creator><scope>EVB</scope></search><sort><creationdate>20230512</creationdate><title>(+) gamma-lactamase gene cloning and expression method and application thereof</title><author>WANG CONG ; LIU CHUNYING ; CHI XUEMEI ; XIA SHUIYING ; CHI NAIYU ; YU SHUANG ; GUO XINZHI ; YUAN WENTAO ; ZHANG QINGFANG ; WANG YAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116103321A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG CONG</creatorcontrib><creatorcontrib>LIU CHUNYING</creatorcontrib><creatorcontrib>CHI XUEMEI</creatorcontrib><creatorcontrib>XIA SHUIYING</creatorcontrib><creatorcontrib>CHI NAIYU</creatorcontrib><creatorcontrib>YU SHUANG</creatorcontrib><creatorcontrib>GUO XINZHI</creatorcontrib><creatorcontrib>YUAN WENTAO</creatorcontrib><creatorcontrib>ZHANG QINGFANG</creatorcontrib><creatorcontrib>WANG YAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG CONG</au><au>LIU CHUNYING</au><au>CHI XUEMEI</au><au>XIA SHUIYING</au><au>CHI NAIYU</au><au>YU SHUANG</au><au>GUO XINZHI</au><au>YUAN WENTAO</au><au>ZHANG QINGFANG</au><au>WANG YAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>(+) gamma-lactamase gene cloning and expression method and application thereof</title><date>2023-05-12</date><risdate>2023</risdate><abstract>The invention belongs to the field of gene engineering, and discloses a cloning and expression method and application of a (+) gamma-lactamase gene. A gene for producing (+) gamma-lactamase is explored by adopting a strain, target gene amplification is carried out on the gene, and an engineering bacterium is constructed for producing (+) gamma-lactamase, so that industrial production of (-) gamma-lactam is carried out.
本发明属于基因工程领域，公开了一种(+)γ-内酰胺酶基因的克隆与表达方法及其应用。采用菌株发掘出产(+)γ-内酰胺酶的基因，将其进行目的基因扩增，构建工程菌，用来生产(+)γ-内酰胺酶，从而进行工业化生产(-)γ-内酰胺。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | (+) gamma-lactamase gene cloning and expression method and application thereof |
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