(+) gamma-lactamase gene cloning and expression method and application thereof

The invention belongs to the field of gene engineering, and discloses a cloning and expression method and application of a (+) gamma-lactamase gene. A gene for producing (+) gamma-lactamase is explored by adopting a strain, target gene amplification is carried out on the gene, and an engineering bac...

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Bibliographische Detailangaben
Hauptverfasser:	WANG CONG, LIU CHUNYING, CHI XUEMEI, XIA SHUIYING, CHI NAIYU, YU SHUANG, GUO XINZHI, YUAN WENTAO, ZHANG QINGFANG, WANG YAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of gene engineering, and discloses a cloning and expression method and application of a (+) gamma-lactamase gene. A gene for producing (+) gamma-lactamase is explored by adopting a strain, target gene amplification is carried out on the gene, and an engineering bacterium is constructed for producing (+) gamma-lactamase, so that industrial production of (-) gamma-lactam is carried out. 本发明属于基因工程领域，公开了一种(+)γ-内酰胺酶基因的克隆与表达方法及其应用。采用菌株发掘出产(+)γ-内酰胺酶的基因，将其进行目的基因扩增，构建工程菌，用来生产(+)γ-内酰胺酶，从而进行工业化生产(-)γ-内酰胺。