Purification method of human mitochondrial respiratory chain compound II

The invention belongs to the technical field of protein purification, and particularly relates to a purification method of a human mitochondrial respiratory chain compound II. The purification method provided by the invention comprises the following steps: filling a human mitochondrial complex total protein solution into a chromatographic column filled with Anti-Flag filler, carrying out affinity column chromatography purification, filling an obtained human mitochondrial respiratory chain complex II crude extract into a gel exclusion chromatographic column, and carrying out gel exclusion column chromatography purification, carrying out second elution by adopting a second eluent, and then collecting second eluent to obtain a pure product of the human mitochondrial respiratory chain compound II, a 3 * Flag tag is fused at the C end of the SDHD subunit of the human mitochondrial respiratory chain complex II in the human mitochondrial complex total protein solution. The purification method provided by the inventi