Angelica sinensis tissue culture seedling rooting culture method

The invention provides an angelica sinensis tissue culture seedling rooting culture method, and belongs to the technical field of agricultural biologication.The angelica sinensis tissue culture seedling rooting culture method comprises the steps that a rootless seedling with the height of 1-3 cm is cut into single buds under the sterile condition, the single buds are transferred into a rooting culture medium to be subjected to rooting induction under the set culture condition, and test-tube plantlet seedlings are obtained; the seedling hardening time is one week, CO2 is released in a seedling hardening chamber in the seedling hardening process, the concentration of the CO2 is gradually increased, then a culture medium of the seedling subjected to seedling hardening is washed away, the seedling is transplanted into a transplanting medium, the seedling is cultured till new buds start to be extracted, then the seedling is placed in an outdoor shade place to grow, and seedling hardening and transplanting are cond