Construction method of self-powered sensor based on nano-enzyme signal amplification strategy and application of self-powered sensor to detection of microcystin-LR

The invention belongs to the technical field of photoelectrochemical sensors, and discloses a construction method of a self-powered sensor based on a nano-enzyme signal amplification strategy and application of the self-powered sensor to detection of microcystin-LR. The method comprises the following steps: step 1, preparing photoactive materials ZnO NRs/FTO and CuS/FTO; 2, Co3O4HNPs modified by an aptamer is prepared, and Co3O4HNPs modified by the aptamer is prepared; and step 3, constructing the self-powered sensor based on nano-enzyme induced signal amplification. The sensor does not need an external power supply, and is high in light energy utilization rate and high in sensitivity. The prepared Co3O4HNPs not only can compete for light energy with a photo-anode, but also can induce an enzymatic reaction to generate insoluble biological precipitates on the surface of an electrode, so that electrons of the photo-anode are prevented from being transferred to a photo-cathode, and amplification of response sign