Preparation method and application of sarcosine oxidase

The invention discloses a preparation method and application of sarcosine oxidase. Compared with other bacteria sarcosine oxidase or optimized codons obtained by different optimization modes of the bacillus sarcosine oxidase, the bacillus sarcosine oxidase optimized codon provided by the invention has the advantages that the soluble protein expression amount in escherichia coli is high, and the enzyme activity of an expression product is high; the sarcosine oxidase expression method of the prokaryotic expression system is high in yield, simple in purification step and suitable for industrial production. 本申请公开了一种肌氨酸氧化酶的制备方法和应用。本申请中提供的芽孢杆菌属肌氨酸氧化酶优化密码子，相比其他菌属肌氨酸氧化酶或者芽孢杆菌属肌氨酸氧化酶经不同优化方式得到的优化密码子，在大肠杆菌中表达可溶蛋白量高，且表达产物酶活高；本申请中提供的原核表达体系的肌氨酸氧化酶表达方法，收率高，纯化步骤简单，适宜工业化生产。