Preparation method of high-purity kaempferol 3-O-(2 ''-O-apiperidinyl) rutin

The invention discloses a preparation method of high-purity kaempferol 3-O-(2 ''-O-apiprafuryl) rutin. The preparation method comprises the following steps: extracting medicinal materials; injecting the extracting solution into a preparative liquid chromatograph, taking acetonitrile-1% glacial acetic acid as a mobile phase, and collecting 10-11 minutes effluent to obtain primary preparative eluent; recovering the solvent from the obtained eluent, dissolving residues with a proper amount of methanol, feeding the obtained solution into a high performance liquid chromatograph, and collecting the effluent for 5-7 minutes by taking acetonitrile-1% glacial acetic acid as a mobile phase to obtain a secondarily prepared eluent; and recovering the solvent from the eluent, and drying under reduced pressure to obtain a target compound. According to the method, the target compound can be efficiently, rapidly and pertinently separated from the folium sennae auriculati, the purity is high (greater than 98%), and the yield