Composite adjuvant, vaccine and preparation method

The invention provides a composite adjuvant, a vaccine and a preparation method, and belongs to the technical field of vaccines. The composite adjuvant is prepared from the following components in parts by mass: (5-100) * 10 parts of CpG-ODN, 0.2-1.2 parts of aluminum hydroxide and 1-5 parts of glycine. The CpG-ODN and the aluminum hydroxide in the composite adjuvant have excellent antibody titer improving capacity, and the glycine can improve the stability of the antigen. After the EV71 inactivated virus is mixed with the composite adjuvant disclosed by the invention, the EV71 inactivated virus is stored for 180 days at 4 DEG C, the content of the EV71 inactivated virus is only reduced by 5-7%, and the content of the EV71 inactivated virus in a glycine-free group is reduced by 10-20%. 本发明提供了一种复合佐剂、疫苗及制备方法，属于疫苗技术领域；所述复合佐剂包括以下质量份的组分：CpG-ODN(5～100)×10-3份、氢氧化铝0.2～1.2份和甘氨酸1～5份。本发明的复合佐剂中CpG-ODN和氢氧化铝具有优异的抗体效价提高能力，甘氨酸能够提高抗原的稳定性。将EV71灭活病毒与本发明的复合佐剂混合后，EV71灭活病毒在4℃条件下保存180天，EV71灭活病毒的含量仅下降了5％～7％，而无甘氨酸组EV71灭活病毒的含量下降