Digital microfluidic device and high-resolution melting curve analysis method

The invention discloses a digital microfluidic device and a high-resolution melting curve analysis method, and relates to the technical field of digital microfluidics. The invention provides a functional substrate with a graded micro-nano structure, which is used for carrying out high-resolution nL-grade thin-layer liquid drop separation and arraying on a digital micro-fluidic platform so as to realize accurate one-time melting curve analysis. Enabling the functional substrate to have a contact angle contrast gt through etching treatment; and the super wettability at 170 DEG C is realized. On a super-hydrophilic pattern array with a unit gap of 50-500 [mu] m, a [mu] L-level DNA sample is passively separated into high-resolution nL-level thin-layer liquid drops, the characterization thickness of the liquid drops is 50 [mu] m, the temperature difference in the liquid drops is greatly reduced, and the method can be used for high-resolution melting curve analysis. In addition, compared with the traditional digita