Electrochemiluminescence sensor for detecting acute promyelocyte PML/RAR alpha fusion gene based on carbon dot and Au-coated Ag2S resonance energy transfer

The invention discloses an electrochemiluminescence sensor for detecting an acute promyelocyte PML/RAR alpha fusion gene based on resonance energy transfer of carbon dots and Au and Ag2S. Carbon dots are adopted as an electrochemiluminescence reagent, CDs are modified on the surface of an electrode...

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Bibliographische Detailangaben
Hauptverfasser:	LIU AILIN, LEI YUN, HAN SHUHUA, ZHANG ZIYANG, ZHANG YU, LIN MUTU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an electrochemiluminescence sensor for detecting an acute promyelocyte PML/RAR alpha fusion gene based on resonance energy transfer of carbon dots and Au and Ag2S. Carbon dots are adopted as an electrochemiluminescence reagent, CDs are modified on the surface of an electrode by forming a CDs/PAMAM/reduced graphene compound (CDs/PAMAM/rGO), the ECL signal of the CDs is efficiently quenched by utilizing resonance energy transfer of Au and Ag2S nanoparticles (Au and Ag2S NPs) to the ECL signal of the CDs, a capture DNA probe is combined with target DNA, and an Au and Ag2S NPs marker assists DNA probe hybridization to form a sandwich structure, so that the CDs/PAMAM/rGO/CDs/PAMAM/rGO/CDs/PAMAM/rGO/CDs/PAMAM/rGO/CDs/PAMAM/rGO/CDs/PAMAM/rGO/CDs/PAMAM/rGO The ECL biosensor for acute promyelocytic leukemia PML/RAR alpha fusion gene detection is established, and the constructed ECL biosensor has the advantages of simplicity, convenience, low cost, high sensitivity and the like, and is expected