Human immunodeficiency virus type 1 nucleic acid determination kit and operation method thereof

The invention relates to a human immunodeficiency virus type 1 nucleic acid determination kit and an operation method thereof. The kit comprises three sets of primers and probes aiming at the following conserved regions: a Gag region, a Pol region and an Ltr region; the specific sequences of the three sets of primers and probes are the same as those in the specification. The detection range of the kit provided by the embodiment of the invention can cover main genotypes in China, and the phenomenon of missing detection in HIV nucleic acid detection can be avoided; the kit is high in detection sensitivity and low in production cost. 一种人类免疫缺陷病毒1型核酸测定试剂盒及其操作方法，所述试剂盒包括针对下述保守区的三套引物和探针：Gag区、Pol区和Ltr区；三套引物和探针的具体序列与说明书相同。本公开实施例提供的试剂盒的检测范围能够覆盖中国国内的主要基因型别，可以避免HIV核酸检测中漏检现象的发生；试剂盒的检测灵敏度高，生产成本低。