Method and kit for rapidly detecting deep infection aspergillus

The method comprises the following steps: mixing a to-be-detected sample with a nucleic acid direct-cracking extracting solution, heating at 60-65 DEG C for 8-20 minutes, heating at 93-98 DEG C for 5-10 minutes to obtain a DNA extracting solution of the to-be-detected sample, adding the DNA extracti...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG JUNYAN, CUI LAN, LEI CHENGGANG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The method comprises the following steps: mixing a to-be-detected sample with a nucleic acid direct-cracking extracting solution, heating at 60-65 DEG C for 8-20 minutes, heating at 93-98 DEG C for 5-10 minutes to obtain a DNA extracting solution of the to-be-detected sample, adding the DNA extracting solution into a PCR premixed solution of a composition containing the deep-infected aspergillus to prepare a PCR reaction solution, and carrying out quantitative detection on the deep-infected aspergillus by using the PCR reaction solution. The PCR reaction liquid is subjected to the following PCR amplification reactions: denaturation is carried out when the temperature is controlled to be 90-100 DEG C, the retention time is 0-5 s, annealing extension is carried out when the temperature is controlled to be 50-70 DEG C, the retention time is 0-5 s, and circulation is carried out 35-50 times. According to the method, the specific primer probe composition capable of distinguishing the aspergillus flavus, the asperg