Construction method of high-efficiency sequencing library, primer group and kit

The invention discloses a construction method of a high-efficiency sequencing library, a primer group and a kit. The construction method comprises the following steps: obtaining DNA of a plurality of samples; performing first PCR amplification on the DNA of each sample by using the first primer, the second primer and the third primer; combining amplification products of a plurality of samples into one tube; performing second PCR amplification on the merged amplification product by using a fourth primer and a fifth primer to obtain a sequencing library; the first primer is a first sequencing tag sequence and an amplicon specific forward primer; the second primer is a second sequencing tag sequence and an amplicon specific reverse primer; the third primer comprises a second sequencing linker sequence, a sample tag sequence and a second sequencing tag sequence; the sample tag sequence is used for marking different samples; the fourth primer is a first sequencing linker sequence and a first sequencing tag sequenc