Hemocyte pathogenic microorganism host-removing nucleic acid extraction kit and method

The invention relates to the technical field of biology, in particular to a blood cell pathogenic microorganism host-removing nucleic acid extraction kit and method, and the blood cell pathogenic microorganism host-removing nucleic acid extraction kit comprises a cell lysis solution, DNA enzyme, a D...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG PENG, YU TONG, ZENG ZHIPENG, XING KUAN, HE GUILUN, LI DONGQING, XIE ZHEN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of biology, in particular to a blood cell pathogenic microorganism host-removing nucleic acid extraction kit and method, and the blood cell pathogenic microorganism host-removing nucleic acid extraction kit comprises a cell lysis solution, DNA enzyme, a DNA enzyme buffer solution, protease K, lysozyme, lywallzyme, glass beads and an extraction lysis solution; the cell lysis solution is a saponin and beta octyl glucosinolate solution; the DNA (deoxyribonucleic acid) enzyme is Ultra Nuclease; the DNA enzyme buffer solution is prepared from MgCl2, Tris-HCl and BSA (Bovine Serum Albumin); low-concentration saponin and low-concentration beta-octyl glucosinolate are adopted, and detection of various pathogenic microorganisms is considered under the condition that a good host removing effect is guaranteed. Wall breaking is carried out on microbial cells through combined use of double effects of a chemical method and a physical method, the positive detection rate of pathog