Preparation method and application of eukaryotic expression vector of Nile tilapia CD36 protein
The invention discloses a preparation method and application of an eukaryotic expression vector of Nile tilapia CD36 protein. The preparation method comprises the following steps: extracting RNA (Ribonucleic Acid) of Nile tilapia intestinal tissues and synthesizing cDNA (Complementary Deoxyribose Nu...
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| creator | HU HUILING XIA LIQUN WANG ZHIWEN NOH EUI-SEON XIA HONGLI XU LUQIAN HUANG ZIWEI |
| description | The invention discloses a preparation method and application of an eukaryotic expression vector of Nile tilapia CD36 protein. The preparation method comprises the following steps: extracting RNA (Ribonucleic Acid) of Nile tilapia intestinal tissues and synthesizing cDNA (Complementary Deoxyribose Nucleic Acid); carrying out PCR (Polymerase Chain Reaction) amplification on a coding sequence fragment of the Nile tilapia CD36 gene to obtain a Nile tilapia CD36 protein gene; and then constructing a eukaryotic expression vector pEGFP-N1-CD36 of the Nile tilapia mossambica CD36 protein. The eukaryotic expression vector of the nucleotide sequence of the Nile tilapia CD36 protein prepared by the invention can be used for preparing a product for improving the anti-streptococcus immunity of tilapia, and the product is simple and easy to prepare and low in cost; the nile tilapia CD36 protein is over-expressed in tilapia bodies to enhance the streptococcus resistance of tilapia, so that the survival rate of tilapia infec |
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The preparation method comprises the following steps: extracting RNA (Ribonucleic Acid) of Nile tilapia intestinal tissues and synthesizing cDNA (Complementary Deoxyribose Nucleic Acid); carrying out PCR (Polymerase Chain Reaction) amplification on a coding sequence fragment of the Nile tilapia CD36 gene to obtain a Nile tilapia CD36 protein gene; and then constructing a eukaryotic expression vector pEGFP-N1-CD36 of the Nile tilapia mossambica CD36 protein. The eukaryotic expression vector of the nucleotide sequence of the Nile tilapia CD36 protein prepared by the invention can be used for preparing a product for improving the anti-streptococcus immunity of tilapia, and the product is simple and easy to prepare and low in cost; the nile tilapia CD36 protein is over-expressed in tilapia bodies to enhance the streptococcus resistance of tilapia, so that the survival rate of tilapia infec</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230505&DB=EPODOC&CC=CN&NR=116064635A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230505&DB=EPODOC&CC=CN&NR=116064635A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HU HUILING</creatorcontrib><creatorcontrib>XIA LIQUN</creatorcontrib><creatorcontrib>WANG ZHIWEN</creatorcontrib><creatorcontrib>NOH EUI-SEON</creatorcontrib><creatorcontrib>XIA HONGLI</creatorcontrib><creatorcontrib>XU LUQIAN</creatorcontrib><creatorcontrib>HUANG ZIWEI</creatorcontrib><title>Preparation method and application of eukaryotic expression vector of Nile tilapia CD36 protein</title><description>The invention discloses a preparation method and application of an eukaryotic expression vector of Nile tilapia CD36 protein. The preparation method comprises the following steps: extracting RNA (Ribonucleic Acid) of Nile tilapia intestinal tissues and synthesizing cDNA (Complementary Deoxyribose Nucleic Acid); carrying out PCR (Polymerase Chain Reaction) amplification on a coding sequence fragment of the Nile tilapia CD36 gene to obtain a Nile tilapia CD36 protein gene; and then constructing a eukaryotic expression vector pEGFP-N1-CD36 of the Nile tilapia mossambica CD36 protein. The eukaryotic expression vector of the nucleotide sequence of the Nile tilapia CD36 protein prepared by the invention can be used for preparing a product for improving the anti-streptococcus immunity of tilapia, and the product is simple and easy to prepare and low in cost; the nile tilapia CD36 protein is over-expressed in tilapia bodies to enhance the streptococcus resistance of tilapia, so that the survival rate of tilapia infec</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNijEKwkAQRdNYiHqH8QCCIbq9rIpVsLAPwzrBwXVn2B1Fb69BD2DxefDeH1fdMZNiRmNJcCO7yBkwfaYaOXy19ED3K-aXGAegp2YqZQgPCiZ56C1HAuOIygh-2zjQLEacptWox1ho9uOkmu93J39YkEpHRTFQIut8W9du6VauWW-afz5viNo8vQ</recordid><startdate>20230505</startdate><enddate>20230505</enddate><creator>HU HUILING</creator><creator>XIA LIQUN</creator><creator>WANG ZHIWEN</creator><creator>NOH EUI-SEON</creator><creator>XIA HONGLI</creator><creator>XU LUQIAN</creator><creator>HUANG ZIWEI</creator><scope>EVB</scope></search><sort><creationdate>20230505</creationdate><title>Preparation method and application of eukaryotic expression vector of Nile tilapia CD36 protein</title><author>HU HUILING ; XIA LIQUN ; WANG ZHIWEN ; NOH EUI-SEON ; XIA HONGLI ; XU LUQIAN ; HUANG ZIWEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116064635A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HU HUILING</creatorcontrib><creatorcontrib>XIA LIQUN</creatorcontrib><creatorcontrib>WANG ZHIWEN</creatorcontrib><creatorcontrib>NOH EUI-SEON</creatorcontrib><creatorcontrib>XIA HONGLI</creatorcontrib><creatorcontrib>XU LUQIAN</creatorcontrib><creatorcontrib>HUANG ZIWEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HU HUILING</au><au>XIA LIQUN</au><au>WANG ZHIWEN</au><au>NOH EUI-SEON</au><au>XIA HONGLI</au><au>XU LUQIAN</au><au>HUANG ZIWEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation method and application of eukaryotic expression vector of Nile tilapia CD36 protein</title><date>2023-05-05</date><risdate>2023</risdate><abstract>The invention discloses a preparation method and application of an eukaryotic expression vector of Nile tilapia CD36 protein. The preparation method comprises the following steps: extracting RNA (Ribonucleic Acid) of Nile tilapia intestinal tissues and synthesizing cDNA (Complementary Deoxyribose Nucleic Acid); carrying out PCR (Polymerase Chain Reaction) amplification on a coding sequence fragment of the Nile tilapia CD36 gene to obtain a Nile tilapia CD36 protein gene; and then constructing a eukaryotic expression vector pEGFP-N1-CD36 of the Nile tilapia mossambica CD36 protein. The eukaryotic expression vector of the nucleotide sequence of the Nile tilapia CD36 protein prepared by the invention can be used for preparing a product for improving the anti-streptococcus immunity of tilapia, and the product is simple and easy to prepare and low in cost; the nile tilapia CD36 protein is over-expressed in tilapia bodies to enhance the streptococcus resistance of tilapia, so that the survival rate of tilapia infec</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Preparation method and application of eukaryotic expression vector of Nile tilapia CD36 protein |
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