Preparation method and application of eukaryotic expression vector of Nile tilapia CD36 protein

The invention discloses a preparation method and application of an eukaryotic expression vector of Nile tilapia CD36 protein. The preparation method comprises the following steps: extracting RNA (Ribonucleic Acid) of Nile tilapia intestinal tissues and synthesizing cDNA (Complementary Deoxyribose Nucleic Acid); carrying out PCR (Polymerase Chain Reaction) amplification on a coding sequence fragment of the Nile tilapia CD36 gene to obtain a Nile tilapia CD36 protein gene; and then constructing a eukaryotic expression vector pEGFP-N1-CD36 of the Nile tilapia mossambica CD36 protein. The eukaryotic expression vector of the nucleotide sequence of the Nile tilapia CD36 protein prepared by the invention can be used for preparing a product for improving the anti-streptococcus immunity of tilapia, and the product is simple and easy to prepare and low in cost; the nile tilapia CD36 protein is over-expressed in tilapia bodies to enhance the streptococcus resistance of tilapia, so that the survival rate of tilapia infec