Cellulase gene, cellulase, recombinant vector and application

The invention discloses a cellulase gene, cellulase, a recombinant vector and application, the sequence of the cellulase gene is as shown in SEQ ID NO.1 or SEQ ID NO.2, or the nucleotide sequence is obtained after site mutation of the nucleotide sequence as shown in SEQ ID NO.1 or SEQ ID NO.2, the substrate binding domain of the cellulase is PKD-Fn3, and the nucleotide sequence of the KD-Fn3 is as shown in SEQ ID NO.3. A metagenomics technology is utilized to excavate and research novel and efficient cellulase genes in a Bemuramah soil metagenomic library, and after the screened cellulase genes are subjected to heterologous expression and protein purification to obtain cellulase, the enzymatic characteristics and dynamic properties of the cellulase are analyzed. The novel and efficient cellulase screened by the research not only can enrich the existing cellulase resource library, but also can provide a reference basis for improving the utilization efficiency of lignocellulose. 本发明公开了一种纤维素酶基因、纤维素酶、重组载体及应用，纤维素酶