Specific DNA fragment for sex identification of mastacembelus armatus, genetic sex marking primer and application

The invention discloses a specific DNA fragment for sex identification of mastacembelus armatus, a genetic sex marking primer and application. The specific DNA fragment is one of the following primers: a Contig-1 upstream primer, a Contig-1 downstream primer, a Contig-1 upstream primer, a Contig-1 downstream primer and a Contig-1 upstream primer, the nucleotide sequence of the Contig-1 downstream primer is as shown in SEQ ID NO. 2; the nucleotide sequence of the Contig-2 upstream primer is as shown in SEQ ID NO. 3; the nucleotide sequence of the Contig-2 downstream primer is as shown in SEQ ID NO. 4; the genetic sex marker primer for sex identification of the mastacembelus armatus is a primer pair 1 and comprises a Contig-1 upstream primer and a Contig-1 downstream primer. The specific DNA fragment and the genetic sex marker primer are applied to all-male breeding of the mastacembelus armatus. The method is wide in application range, short in time consumption and accurate in detection result. 本发明公开了一种大刺鳅性别鉴定的