Aflatoxin oxidase for improving pepsin resistance

The invention discloses aflatoxin oxidase (AFO) for improving pepsin resistance and application of the aflatoxin oxidase (AFO). Key amino acid residues in wild aflatoxin oxidase molecules are modified through a protein engineering technology, and the mutant aflatoxin oxidase capable of improving pep...

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Hauptverfasser:	HUANG JIONGWEI, GUO MENGYING, MO SHIYI, QIAN DAN, LIU DALING, YAO DONGSHENG, LIU GUIZHEN, XIE CHUNFANG, LAO WEIMING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses aflatoxin oxidase (AFO) for improving pepsin resistance and application of the aflatoxin oxidase (AFO). Key amino acid residues in wild aflatoxin oxidase molecules are modified through a protein engineering technology, and the mutant aflatoxin oxidase capable of improving pepsin resistance is obtained through screening. The resistance half-life period of the mutant aflatoxin oxidase (AFOP477N) acting on aflatoxin (AFB1) to pepsin is prolonged by 22% compared with that of wild type AFO. Compared with a wild type, the mutant AFOP477N still has more than 60% of activity at the temperature of 20-60 DEG C; the optimal reaction pH is 6.0; and when the pH value is 7.0-8.0, the activity is kept by more than 40%. 本发明公开了一种提高胃蛋白酶抗性的黄曲霉毒素氧化酶(AFO)及其应用。本发明通过蛋白质工程技术对野生型黄曲霉毒素氧化酶分子内的关键氨基酸残基进行了改造，筛选得到了一种提高胃蛋白酶抗性的突变体黄曲霉毒素氧化酶。本发明所述的作用于黄曲霉毒素(AFB1)的突变体黄曲霉毒素氧化酶(AFOP477N)对胃蛋白酶的抗性半衰期比野生型AFO延长了22％。突变体AFOP477N相比于野生型，在20～60℃时仍保有60％以上的活性；最适反应pH为6.0；在pH 7.0～8.0时保有40％以上的活性。