Multispecific antigen binding proteins

The present invention provides, inter alia, multispecific antigen binding proteins or antigen binding fragments thereof comprising one or more mutations in the VH/VL domain and/or CH1/CL domain, pharmaceutical compositions comprising the multispecific antigen binding proteins or antigen binding fragments thereof, isolated nucleic acids, vectors and host cells encoding/expressing the multispecific antigen binding proteins or antigen binding fragments thereof, methods of making the multispecific antigen binding proteins, computer readable media and libraries for evaluating the multispecific antigen binding proteins. 本发明尤其提供在VH/VL结构域和/或CH1/CL结构域中包含一个或多个突变的多特异性抗原结合蛋白或其抗原结合片段，包含该多特异性抗原结合蛋白或其抗原结合片段的药物组合物，编码/表达该多特异性抗原结合蛋白或其抗原结合片段的分离的核酸、载体和宿主细胞，制备多特异性抗原结合蛋白的方法，用于评价多特异性抗原结合蛋白的计算机可读介质及文库。