Universal monoclonal antibody HOPE1 for resisting natural capsaicin, dihydrocapsaicin and synthetic capsaicin and application of universal monoclonal antibody HOPE1

Universal monoclonal antibody HOPE1 for resisting natural capsaicin, dihydrocapsaicin and synthetic capsaicin and application of universal monoclonal antibody HOPE1

The invention discloses a universal monoclonal antibody HOPE1 for resisting natural capsaicin, dihydrocapsaicin and synthetic capsaicin and application of the universal monoclonal antibody HOPE1. The sequence of a heavy chain variable region of the universal monoclonal antibody HOPE1 is as shown in...

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Bibliographische Detailangaben
Hauptverfasser: LEI HONGTAO, SHEN YUDONG, CHEN RUNXI, WANG ZHIRUO, LUO LIN, WANG YUPU, CHEN ZIJIAN, XU ZHENLIN, WEN WEIHUAN, ZENG HAILI
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a universal monoclonal antibody HOPE1 for resisting natural capsaicin, dihydrocapsaicin and synthetic capsaicin and application of the universal monoclonal antibody HOPE1. The sequence of a heavy chain variable region of the universal monoclonal antibody HOPE1 is as shown in SEQ ID NO.1, and the sequence of a light chain variable region of the universal monoclonal antibody HOPE1 is as shown in SEQ ID NO.2. The half inhibition concentrations of the monoclonal antibody HOPE1 to natural capsaicin, synthetic capsaicin, dihydrocapsaicin and a mixture of the natural capsaicin, the synthetic capsaicin and the dihydrocapsaicin respectively reach 0.32 ng/mL, 0.07 ng/mL, 0.21 ng/mL and 103.8 pg/mL, and the monoclonal antibody HOPE1 has strong affinity to capsaicin substances; an ic-ELISA (Enzyme-Linked Immunosorbent Assay) immunodetection method and a time-resolved fluorescence immunochromatography method, which are established by the monoclonal antibody HOPE1 provided by the invention and are u