Mutant of rabies virus G protein and application thereof
The invention discloses a rabies virus G protein mutant and application thereof, and belongs to the technical field of biology. The mutant of the rabies virus G protein provided by the invention is obtained by mutating the 313th amino acid of the wild type rabies virus G protein from lysine to argin...
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| creator | LI LIYAN LU XIAOYU LU WENYAN ZHENG JINLAI CHEN GUOQING DU HAOYUN LI TIANZENG FAN JUNWEN XU YUANYUAN GAO LONG ZHENG XUEYING DENG BAILIN ZHANG HUI ZHAO BING ZHAO SONG REN LISONG LYU LUJIE HAO JINBAO |
| description | The invention discloses a rabies virus G protein mutant and application thereof, and belongs to the technical field of biology. The mutant of the rabies virus G protein provided by the invention is obtained by mutating the 313th amino acid of the wild type rabies virus G protein from lysine to arginine, mutating the 409th amino acid from serine to cysteine and optionally replacing a signal peptide of the wild type rabies virus G protein. After mutation of two sites and optional replacement of the signal peptide, the stability of the mutant of the rabies virus G protein and the expression quantity of the mutant in a eukaryotic expression system are obviously improved. The rabies virus G protein mutant provided by the invention can be used for accurately detecting the concentration of the rabies virus antibody in serum, and is good in specificity, so that the rabies virus G protein mutant can be applied to quantitative detection of the rabies virus antibody, and the immune effect of a rabies virus vaccine can b |
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The mutant of the rabies virus G protein provided by the invention is obtained by mutating the 313th amino acid of the wild type rabies virus G protein from lysine to arginine, mutating the 409th amino acid from serine to cysteine and optionally replacing a signal peptide of the wild type rabies virus G protein. After mutation of two sites and optional replacement of the signal peptide, the stability of the mutant of the rabies virus G protein and the expression quantity of the mutant in a eukaryotic expression system are obviously improved. The rabies virus G protein mutant provided by the invention can be used for accurately detecting the concentration of the rabies virus antibody in serum, and is good in specificity, so that the rabies virus G protein mutant can be applied to quantitative detection of the rabies virus antibody, and the immune effect of a rabies virus vaccine can b</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230505&DB=EPODOC&CC=CN&NR=116063410A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230505&DB=EPODOC&CC=CN&NR=116063410A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI LIYAN</creatorcontrib><creatorcontrib>LU XIAOYU</creatorcontrib><creatorcontrib>LU WENYAN</creatorcontrib><creatorcontrib>ZHENG JINLAI</creatorcontrib><creatorcontrib>CHEN GUOQING</creatorcontrib><creatorcontrib>DU HAOYUN</creatorcontrib><creatorcontrib>LI TIANZENG</creatorcontrib><creatorcontrib>FAN JUNWEN</creatorcontrib><creatorcontrib>XU YUANYUAN</creatorcontrib><creatorcontrib>GAO LONG</creatorcontrib><creatorcontrib>ZHENG XUEYING</creatorcontrib><creatorcontrib>DENG BAILIN</creatorcontrib><creatorcontrib>ZHANG HUI</creatorcontrib><creatorcontrib>ZHAO BING</creatorcontrib><creatorcontrib>ZHAO SONG</creatorcontrib><creatorcontrib>REN LISONG</creatorcontrib><creatorcontrib>LYU LUJIE</creatorcontrib><creatorcontrib>HAO JINBAO</creatorcontrib><title>Mutant of rabies virus G protein and application thereof</title><description>The invention discloses a rabies virus G protein mutant and application thereof, and belongs to the technical field of biology. 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The mutant of the rabies virus G protein provided by the invention is obtained by mutating the 313th amino acid of the wild type rabies virus G protein from lysine to arginine, mutating the 409th amino acid from serine to cysteine and optionally replacing a signal peptide of the wild type rabies virus G protein. After mutation of two sites and optional replacement of the signal peptide, the stability of the mutant of the rabies virus G protein and the expression quantity of the mutant in a eukaryotic expression system are obviously improved. The rabies virus G protein mutant provided by the invention can be used for accurately detecting the concentration of the rabies virus antibody in serum, and is good in specificity, so that the rabies virus G protein mutant can be applied to quantitative detection of the rabies virus antibody, and the immune effect of a rabies virus vaccine can b</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Mutant of rabies virus G protein and application thereof |
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